Learn how to overcome the problems of quantitative proteomics experiments

ABRF 2012 takes place at Disney’s Contemporary ResortFollowing on from Beth’s last blog post I have more details of our Tutorial Session (T5), which is being held on Tuesday 20th March (4:30pm -5:45pm) at the ABRF 2012 meeting in Orlando, Florida.

The title of the tutorial session is Overcoming problems with experiment design for quantitative proteomics and, after a call with all the speakers on Friday, it is shaping up to be a great set of talks.

ABRF 2012 takes place at Disney’s Contemporary Resort

What you will hear at our tutorial session

The brief from the ABRF organising committee was to “provide a session with speakers that would highlight the challenges of experiment design and the statistical analysis of ‘omics data.” They also said “people should leave the session having learnt something they can take back to their lab, share and apply to their research.” So, we have organised the talks into a sequence, adding more detail to the subject as each one is presented.

We’ll start with a high level view from Will Dracup, Chairman and founder of Nonlinear Dynamics, on the major challenges you face in designing and running good proteomics experiments. This will include some fundamental concepts you need to bear in mind from the start and what a good proteomics experiment means.

Following this, Julian Whitelegge Ph.D. who leads the proteomics division of the Pasarow Mass Spectrometry Laboratory at UCLA, will talk on introducing inexperienced users to label-free quantitative proteomics. He will share their experiences in helping to transition facility users and collaborators from protein identification experiments to quantitative LC-MS proteomics experiments. 

Finally, we will have a talk by Juan Chavez, Ph.D. from The Bruce Lab at the University of Washington, a lab that focuses on development of advanced mass spectrometry technology for proteomics, biological and biomedical applications. This presentation will focus on a SILAC experiment to measure expression level differences between drug resistant and drug sensitive HeLa cells. The example will demonstrate good experiment design and how to approach statistical analysis of ‘omics data.

RSS feed for Nonlinear Dynamics blog@NonlinearDotComI’ll be tweeting (follow @NonlinearDotCom) before and during the event with any interesting developments. A blog post reviewing the ABRF 2012 meeting and our tutorial session will follow. So be sure to connect with us and find out more.

If you still haven’t registered to attend ABRF 2012, the details are here. It would be great to meet you at our booth or at the tutorial session if you do attend.

ABRF 2012